Analyzing partially randomized nucleic acid pools: straight dope on doping

Analyzing partially randomized nucleic acid pools: straight dope on doping.

Partially randomized (doped) pools are important for optimizing activities initially isolated by selection-amplification or SELEX, and for locating nucleotides critical for function. Here we present a method for calculating the number of unique sequences in a pool, and the expected copy number of each unique sequence with a specified number of changes from the original sequence. Surprisingly, s...

Running Head: Doping Perceptions and Willingness to Dope

To enable preventive measures to be designed, it is important to identify modifiable distal and proximal factors underlying doping behaviour. This study investigated aspects of the prototype willingness model in relation to doping. A cross-sectional study was conducted involving 729 competitive athletes. Following ethical approval, athletes (mean age= 28.8 ± 10.1 years; 63% male) completed an o...

Analyzing Protein - Nucleic Acid Complexes using Hybrid Methods

Electrophoretic Mobility Shift Assay: Analyzing Protein – Nucleic Acid Interactions

The characterization of protein-nucleic acid interactions is essential not only for understanding the wide range of cellular processes they are involved in, but also the mechanisms underlying numerous diseases associated with the breakdown of regulatory systems. These include, but are far from being limited to, cell cycle disorders such as cancer and those caused by pathogenic agents that rely ...

On Thymus Nucleic Acid

A consideration of the earlier investigations of the nucleic acids would lead us to the conclusion that these substances yield among their hydrolytic products four purin derivatives, namely, xanthin, hypoxanthin, guanin, and adenin. Of these four bases hypoxanthin was the first discovered in this connection and was for a long time looked upon as the most constant in its occurrence, and, therefo...